control apc-labeled rat igg isotype  (Thermo Fisher)

Journal: International Journal of Molecular Sciences

Article Title: Therapeutic Anti-KIR Antibody of 1–7F9 Attenuates the Antitumor Effects of Expanded and Activated Human Primary Natural Killer Cells on In Vitro Glioblastoma-like Cells and Orthotopic Tumors Derived Therefrom

doi: 10.3390/ijms241814183

Figure Lengend Snippet: ( A ) KIR2DL1, KIRDL2, and KIR2DL3 expression patterns in GBM from the GlioVis data portal and TCGA database. Left : KIR2DL1 and KIR2DL2 were not significantly expressed in GBM tissues as compared to normal brain tissue in TCGA database ( p = 0.08 and 0.69, respectively), while KIR2DL3 was significantly lower expressed in GBM ( p < 0.01). Right : Kaplan-Meier curves based on mRNA expression from GlioVis data portal and TCGA database. The p -value was determined using Tukey’s honest significant difference test. Low KIR2DL1 expression predicted poor OS with significant differences in TCGA database ( p = 0.04), while KIR2DL2 and KIR2DL3 expression did not ( p = 0.29, and 0.33, respectively). * p < 0.05. ( B ) KIR2DL receptors expression pattern on GiNKs. Left : Representative flow cytometric data of KIR2DL1 and KIRDL2/3 expression on the GiNK surface. The top row was calculated based on the fluorescent intensity of APC-labeled IgG as an isotype control (Ctrl). The middle and bottom row were calculated based on the APC-labeled anti-KIR2DL1 and KIR2DL2/3, respectively. The KIR2DL1 and KIR2DL2/3 expression on the GiNKs were 11.5% and 32.4%, respectively. Right : The frequency of KIR2DL1 + /CD56 + NK cells and KIR2DL2/3 + /CD56 + NK cells were 8.54–17.3% and 21.1–46.8%, respectively, in four healthy volunteers. At least two independent experiments were performed ( n = 8). ( C ) HLA-C expression pattern in glioma from the HPA data set. Left : Fragments per kilobase of transcript sequence per million base pairs sequenced (FKPM) value of HLA-C in gliomas. Right : Kaplan-Meier curves following log-rank testing demonstrating that low HLA-C expression did not predict poor OS in HPA database significantly ( p = 0.20).

Article Snippet: The isotype control was APC-labeled IgG (REA293, Miltenyi Biotech).

Techniques: Expressing, Labeling, Control, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Therapeutic Anti-KIR Antibody of 1–7F9 Attenuates the Antitumor Effects of Expanded and Activated Human Primary Natural Killer Cells on In Vitro Glioblastoma-like Cells and Orthotopic Tumors Derived Therefrom

doi: 10.3390/ijms241814183

Figure Lengend Snippet: The percentage of apoptotic cells in U87MG ( top ) and T98G GBM cells ( bottom ) induced by target cell only; NB, GiNKs pre-incubated with IgG, and anti-KIR2DL1 antibody at 24 h. Left: Representative panels depicting the fluorescence intensity analysis of the PI and annexin V–APC fractions. Target cell only; NB (the left rows), GiNKs pre-incubated with IgG (the middle rows), and anti-KIR2DL1 antibody (the right rows). Right: Bar graphs illustrating the apoptotic cell analysis based on the flow cytometric data of APC-expressing cells. Statistical differences were determined by two-way ANOVA followed by Tukey’s test. **** p < 0.0001, ** p < 0.01, * p < 0.05.

Article Snippet: The isotype control was APC-labeled IgG (REA293, Miltenyi Biotech).

Techniques: Incubation, Fluorescence, Cell Analysis, Expressing